1,068 research outputs found
East Asia’s Development and Sub-regional Economic Zones:A Focus on Multilateral Cross-border Cooperation between Local Governments
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Rapid and convenient quantitative analysis of SARS-CoV-2 RNA in serous saliva with a direct PCR method
Sensitive and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), frequently performed using direct polymerase chain reaction (PCR), is essential for restricting the spread of coronavirus disease 2019 (COVID-19). However, studies evaluating accurate detection are still required. This study evaluated the quantitativeness and sensitivity of the Ampdirect™ 2019-nCoV detection kit, a direct PCR method. Using saliva with or without Tris-buffered saline (TBS) dilution, linearity, and limits of the N1 and N2 regions of SARS-CoV-2 genomic RNA were assessed using EDX SARS-CoV-2 RNA standard dissolved in RNase-free water (RFW). Fluorescence intensities in non-diluted saliva were higher than those in TBS-diluted samples. Linear regression analysis of detected quantification cycle values and spiked standard RNA concentrations showed that the coefficient of determination of the N1 and N2 genes was 0.972 and 0.615 in RFW and 0.947 and 0.660 in saliva, respectively. N1- and N2-positive detection rates in saliva were 46% (6/13 tests) and 0% (0/12 tests) at one copy/reaction, respectively. These results indicate good quantitativeness and sensitivity for N1 but not for N2. Therefore, our findings reveal that the Ampdirect™ 2019-nCoV system, especially targeting the N1 gene, enables rapid and convenient quantification of SARS-CoV-2 RNA in saliva at one copy/reaction
A Design Method for Cascade Form Digital Nyquist Filters with Zero Intersymbol Interference
理工研究域 電子情報学系A method is reported for designing a cascaded Nyquist filter with zero intersymbol interference, particularly when the same transfer function is used in both the transmitter and receiver. The proposed method is applicable to both IIR and FIR filters
Permuted difference coefficients realization of FIR digital filters
金沢大学大学院自然科学研究科情報システム金沢大学工学部new realization for FIR digital filters, using permuted difference coefficients, is proposed. Its coefficients are obtained as the difference between the successive values of the original coefficients reordered in a sequence with falling magnitude. The proposed realization can hold desirable properties in FIR filters, such as an exactly linear phase characteristic and stable implementation, and is effectively applied to a wide range filter response. Quantization error analysis shows that the internal data word lengths must be somewhat increased to maintain the same roundoff noise as in a direct form realization. Computational complexity becomes about 23% and 18% for 99th- and 299th-order filters, taking the excess data word lengths into account, compared with the direct form
A simultaneous frequency and time domain approximation method for discrete-time filters
金沢大学理工研究域 電子情報学
New realization of sum of products and transposed sum of products implementation by partitioned arithmetic
金沢大学大学院自然科学研究科情報システム金沢大学工学部A new approach to the implmentation of sum of products (SP) and transposed sum of products (TSP) using partitioned arithmetic is proposed. This aproach makes it possible to realize SP and TSP with recent advanced semiconductor memory technology without multipliers. It is also shown to offer signficant reductions in memory capacity requirement and computational complexity. Dynamic range constraints and output roundoff noise analysis are also discussed
A discrete optimazation method for high-order FIR filters with finite wordlength coefficients
金沢大学理工研究域 電子情報学
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